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Whereas, a large number of ROS including superoxide (O 2 ), hydroxyl radical (OH ), perhydroxyl radical (HO 2 ), and other types such as hydrogen peroxide (H 2 O 2 ), hypochlorous acid (HOCl), and lipid peroxides (ROOH) [9], is generated as by-products of cellular aerobic metabolism upon different physiological and pathological changes, once exceeding the capacity of the cellular reductase mechanisms, which can result in direct or indirect functional damage to different molecules such as proteins, nucleic acids, and lipids, and eventually lead to cell death [10, 11]

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Updated methods generally require partial revalidation